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dc.contributor.authorKim, Yonghee
dc.contributor.authorAbafogi, Abdurhaman Teyib
dc.contributor.authorTran, Buu Minh
dc.contributor.authorKim, Jaewon
dc.contributor.authorLee, Jinyeop
dc.contributor.authorChen, Zhenzhong
dc.contributor.authorBae, Pan Kee
dc.contributor.authorPark, Kyoungsook
dc.contributor.authorShin, Yong-Beom
dc.contributor.authorNoort, Danny van
dc.contributor.authorLee, Nae Yoon
dc.contributor.authorPark, Sungsu
dc.date.accessioned2021-03-16T23:23:01Z
dc.date.available2021-03-16T23:23:01Z
dc.date.issued2020-02-16
dc.identifier.issn2072-666Xes_PE
dc.identifier.urihttps://hdl.handle.net/20.500.12815/194
dc.description.abstractInfluenzaAviruses are often present in environmental and clinical samples at concentrations below the limit of detection (LOD) of molecular diagnostics. Here we report an integrated microfluidic preconcentration and nucleic amplification system (μFPNAS) which enables both preconcentration of influenza A virus H1N1 (H1N1) and amplification of its viral RNA, thereby lowering LOD for H1N1. H1N1 virus particles were first magnetically preconcentrated using magnetic nanoparticles conjugated with an antibody specific for the virus. Their isolated RNA was amplified to cDNA through thermocycling in a trapezoidal chamber of the μFPNAS. A detection limit as low as 100 TCID50 (50% tissue culture infective dose) in saliva can be obtained within 2 hours. These results suggest that the LOD of molecular diagnostics for virus can be lowered by systematically combining immunomagnetic separation and reverse transcriptase-polymerase chain reaction (RT-PCR) in one microfluidic device.es_PE
dc.formatapplication/pdfes_PE
dc.language.isoenges_PE
dc.publisherMDPIes_PE
dc.rightsinfo:eu-repo/semantics/openAccesses_PE
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.sourceRepositorio Institucional UTECes_PE
dc.sourceUniversidad de Ingeniería y Tecnología - UTECes_PE
dc.subjectMicrofluidic devicees_PE
dc.subjectImmunomagnetic separationes_PE
dc.subjectMolecular amplificationes_PE
dc.subjectH1N1es_PE
dc.titleIntegrated microfluidic preconcentration and nucleic amplification system for detection of influenza a virus H1N1 in salivaes_PE
dc.typeinfo:eu-repo/semantics/articlees_PE
dc.identifier.doihttps://doi.org/10.3390/mi11020203es_PE
dc.identifier.journalMicromachineses_PE


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